Rabbit Anti-bovine IL10 Antibody
Quality Level
100
recombinant
expressed in HEK 293 cells
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RM249, monoclonal
recombinant monoclonal
form
buffered aqueous glycerol solution
species reactivity
human
technique(s)
immunoblotting: 1:1000-1:2000
immunohistochemistry: 1:200-1:500
isotype
IgG
NCBI accession no.
4162
Antibodies that detect IL-10 can be used in several scientific applications, including ELISA, Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin) and Neutralization. These antibodies target IL-10 in Human, Mouse, Rat, Porcine and Bovine samples. Our IL-10 monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Rat, Mouse, Rabbit, Sheep and Goat.
These antibodies have been verified by Neutralization and Cell treatment to confirm specificity to IL-10. Find the IL-10 antibody that fits your needs. Choose from 1 of 76 IL-10 antibodies, which have been validated in experiments with 711 publications and 294 images featured in our data gallery.
Browse primary antibodies for WB, Flow, IHC, ICC/IF, ELISA, IP, and other applications. Antibodies with Advanced Verification data have been validated for specificity to ensure that the antibody binds to the antigen stated. If you cannot find the antibody you're looking for, contact us today to develop custom antibodies for specific targets, species and applications.
Target Information
Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI).
The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF.
IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE).
IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.