Specificity

GenScript His-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing His epitope tags.For N-terminal His-tagged protein detection, THE™ Anti-His mAb (Cat.No.A00186) is recommended.

Applications

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.

Background

His-tag Antibody, pAb, Rabbit recognizes His tagged proteins in transfected or transformed cells.Short-sequence epitope tags are common in the study of protein expression in several different expression systems.In particular, the His tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography.